The Effect of Nickel on the Fibroblast-mediated Collagen Matrix Contraction
نویسندگان
چکیده
We report the effect of nickel chloride on fibroblast-mediated collagen gel contraction, fibroblast migration and pattern of matrix metalloproteinase (MMP) activities expressed by human embrzonary lung fibroblasts (HELFs). After the treatment with nickel chloride, gelatin zymography, casein zymography and also western blot assay revealed a significant decrease of MMP-2 and MMP-1 active forms. MMP-inhibition was associated with a reduction of HELF-mediated collagen matrix contraction. A dose of 10 mM NiCl2 completely suppressed matrix contraction and cell migration.
منابع مشابه
Matrix metalloproteinase inhibition modulates fibroblast-mediated matrix contraction and collagen production in vitro.
PURPOSE To investigate the effect of matrix metalloproteinase (MMP) inhibition on fibroblast-mediated matrix contraction and production. METHODS Free-floating fibroblast-populated type I collagen lattices were prepared with human Tenon's capsule fibroblasts. Lattice areas were photographed and digitally analyzed to indicate the degree of lattice contraction. Quantitative competitive reverse t...
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